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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
13/03/2018 |
Data da última atualização: |
09/05/2018 |
Autoria: |
PEREIRA, A. E.; CARNEIRO, N. P.; SIEGFRIED, B. D. |
Afiliação: |
Adriano E. Pereira, University of Nebraska; NEWTON PORTILHO CARNEIRO, CNPMS; Blair D. Siegfried, University of Florida. |
Título: |
Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Journal of RNAi Gene Silencing, v. 12, p. 528-535, 2016. |
Idioma: |
Inglês |
Conteúdo: |
Corn rootworms (CRW) are the most important corn pests in the U.S. Corn Belt. Damage is caused by larval feeding on the plant roots. RNAi has been studied in CRW and has proven to be effective for the management of this insect with effects on both larval and adult stages. The objective of this study was to determine comparative susceptibility of adult and larva of Southern Corn Rootworm (SCR) and Western Corn Rootworm (WCR) to two different lethal RNAi target genes (vacuolar (v)-ATPase-A and Snf7). Adults and larvae were exposed to dsRNAs overlaid on artificial diet five times every other day. WCR larval susceptibility to vATPase-A was approximately 4-fold more tolerant than SCR, but were similar for Snf7 (2.6 ng/cm2 for both species). For adults, LC50s were generally higher for vATPase-A dsRNA relative to larvae with a 20-fold higher LC50 for WCR (SCR=33.3 ng/cm2; WCR=657.3 ng/cm2) relative to Snf7 dsRNAs (SCR=13.2 ng/cm2; WCR=60.2 ng/cm2). Gene silencing was also evaluated in adults and larvae exposed to the LC50 dsRNAs, and gene knockdown ranged from 48% up to 83% in adults fed for eight days in dsRNA, and from 55% to 89% in larvae fed for 24, 48 and 72 h, compared to control treatments. This is the first study to report LC50 values for dsRNA in WCR and SCR adults. The results indicate that both larvae and adults of both species were affected by vATPase-A and Snf7 dsRNAs suggesting that RNAi for the management of CRW should be considered for both stages. |
Thesagro: |
Gene; Genética; Larva; Praga de planta; Raiz. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/173852/1/Comparative-susceptibility.pdf
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Marc: |
LEADER 02084naa a2200205 a 4500 001 2089001 005 2018-05-09 008 2016 bl uuuu u00u1 u #d 100 1 $aPEREIRA, A. E. 245 $aComparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.$h[electronic resource] 260 $c2016 520 $aCorn rootworms (CRW) are the most important corn pests in the U.S. Corn Belt. Damage is caused by larval feeding on the plant roots. RNAi has been studied in CRW and has proven to be effective for the management of this insect with effects on both larval and adult stages. The objective of this study was to determine comparative susceptibility of adult and larva of Southern Corn Rootworm (SCR) and Western Corn Rootworm (WCR) to two different lethal RNAi target genes (vacuolar (v)-ATPase-A and Snf7). Adults and larvae were exposed to dsRNAs overlaid on artificial diet five times every other day. WCR larval susceptibility to vATPase-A was approximately 4-fold more tolerant than SCR, but were similar for Snf7 (2.6 ng/cm2 for both species). For adults, LC50s were generally higher for vATPase-A dsRNA relative to larvae with a 20-fold higher LC50 for WCR (SCR=33.3 ng/cm2; WCR=657.3 ng/cm2) relative to Snf7 dsRNAs (SCR=13.2 ng/cm2; WCR=60.2 ng/cm2). Gene silencing was also evaluated in adults and larvae exposed to the LC50 dsRNAs, and gene knockdown ranged from 48% up to 83% in adults fed for eight days in dsRNA, and from 55% to 89% in larvae fed for 24, 48 and 72 h, compared to control treatments. This is the first study to report LC50 values for dsRNA in WCR and SCR adults. The results indicate that both larvae and adults of both species were affected by vATPase-A and Snf7 dsRNAs suggesting that RNAi for the management of CRW should be considered for both stages. 650 $aGene 650 $aGenética 650 $aLarva 650 $aPraga de planta 650 $aRaiz 700 1 $aCARNEIRO, N. P. 700 1 $aSIEGFRIED, B. D. 773 $tJournal of RNAi Gene Silencing$gv. 12, p. 528-535, 2016.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
07/12/2009 |
Data da última atualização: |
20/05/2010 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, J. B. da; CARRÃO-PANIZZI, M. C.; PRUDÊNCIO, S. H. |
Afiliação: |
J. B. DA SILVA, UEL; MERCEDES CONCORDIA CARRAO PANIZZI, CNPSo; S. H. PRUDÊNCIO, UEL. |
Título: |
Evaluation of chemical and physical characteristics of Brazilian soybean cultivars for utilization in soy foods. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 253, ref. P670. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng. |
Idioma: |
Inglês |
Palavras-Chave: |
Hexanal; Lipoxigenase; Lipoxygenases; Time cooking. |
Thesagro: |
Aminoácido; Nutrição humana; Propriedade físico-química; Soja. |
Thesaurus NAL: |
Amino acids; Food and Human Nutrition; Soybeans; Sugars. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01175naa a2200277 a 4500 001 1577438 005 2010-05-20 008 2009 bl uuuu u00u1 u #d 100 1 $aSILVA, J. B. da 245 $aEvaluation of chemical and physical characteristics of Brazilian soybean cultivars for utilization in soy foods. 260 $c2009 650 $aAmino acids 650 $aFood and Human Nutrition 650 $aSoybeans 650 $aSugars 650 $aAminoácido 650 $aNutrição humana 650 $aPropriedade físico-química 650 $aSoja 653 $aHexanal 653 $aLipoxigenase 653 $aLipoxygenases 653 $aTime cooking 700 1 $aCARRÃO-PANIZZI, M. C. 700 1 $aPRUDÊNCIO, S. H. 773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 253, ref. P670. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.
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